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Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum Genet. Mol. Biol.
Pinheiro,Fábio; Palma-Silva,Clarisse; Barros,Fábio de; Cozzolino,Salvatore.
In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epidendrum; Orchidaceae; Short tandem repeat; Cross-amplification.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021
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Development and characterization of 20 polymorphic microsatellite markers for Epinephelus marginatus (Lowe, 1834) (Perciformes: Epinephelidae) using 454 pyrosequencing Genet. Mol. Biol.
Vaini,Jussara Oliveira; Mota,Kenneth Gabriel; Ojeda,Alejandra Paola; Barreiros,João Pedro; Moreira,Renata Guimarães; Hilsdorf,Alexandre Wagner Silva.
Abstract The dusky grouper, Epinephelus marginatus, is a well-known and widespread marine fish assessed as endangered by the International Union for the Conservation of Nature. Analyzing the genetic diversity of this species is, therefore, of utmost importance and necessary for conservation purposes. Microsatellites are molecular tools with advantages that are ideal for population analyses. This study provides the first set of species-specific microsatellite loci for E. marginatus that can be applied when assessing both intra- and interpopulation genetic variation. Twenty microsatellite loci were isolated and characterized for the dusky grouper by genotyping 20 individuals obtained from the North Eastern Atlantic Ocean (n = 4) and from the South Western...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Short tandem repeat; Population genetics; Conservation; Fishery; Marine resources.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100074
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